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backbone pjt126  (Addgene inc)


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    Structured Review

    Addgene inc backbone pjt126
    Backbone Pjt126, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/backbone+pjt126/pGEM-sema3B-3'-probe+(Plasmid+%2316192)/bio_rxiv__2024__08__19__608698-367-21-23
    Average 93 stars, based on 15 article reviews
    backbone pjt126 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: High-throughput affinity measurements of direct interactions between activation domains and co-activators
    Article Snippet: .. Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker ( ) using GoldenGate cloning in the backbone pJT126 (Addgene #161926). .. K562 citrine reporter cells (pDY32 Addgene: 161928, stably integrated at the AAVS1 locus) were then transduced with each lentiviral vector and, 3 days later, selected with blasticidin (10 mg/mL) until > 80% of the cells were mCherry positive (6-9 days).

    Article Title: Large-scale mapping and mutagenesis of human transcriptional effector domains.
    Article Snippet: .. Individual recruitment assays and flow cytometry measurements Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker, using GoldenGate cloning in the backbone pJT126 (Addgene no. 161926). .. K562 citrine reporter cells were then transduced with each lentiviral vector and, 3 days later, selected with blasticidin (10 mg ml−1) until more than 80% of the cells were mCherry positive (6–9 days).

    Article Title: Large-scale mapping and systematic mutagenesis of human transcriptional effector domains
    Article Snippet: .. Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker, using GoldenGate cloning in the backbone pJT126 (Addgene #161926). ..

    Marker:

    Article Title: High-throughput affinity measurements of direct interactions between activation domains and co-activators
    Article Snippet: .. Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker ( ) using GoldenGate cloning in the backbone pJT126 (Addgene #161926). .. K562 citrine reporter cells (pDY32 Addgene: 161928, stably integrated at the AAVS1 locus) were then transduced with each lentiviral vector and, 3 days later, selected with blasticidin (10 mg/mL) until > 80% of the cells were mCherry positive (6-9 days).

    Article Title: Large-scale mapping and mutagenesis of human transcriptional effector domains.
    Article Snippet: .. Individual recruitment assays and flow cytometry measurements Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker, using GoldenGate cloning in the backbone pJT126 (Addgene no. 161926). .. K562 citrine reporter cells were then transduced with each lentiviral vector and, 3 days later, selected with blasticidin (10 mg ml−1) until more than 80% of the cells were mCherry positive (6–9 days).

    Article Title: Large-scale mapping and systematic mutagenesis of human transcriptional effector domains
    Article Snippet: .. Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker, using GoldenGate cloning in the backbone pJT126 (Addgene #161926). ..

    Cloning:

    Article Title: High-throughput affinity measurements of direct interactions between activation domains and co-activators
    Article Snippet: .. Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker ( ) using GoldenGate cloning in the backbone pJT126 (Addgene #161926). .. K562 citrine reporter cells (pDY32 Addgene: 161928, stably integrated at the AAVS1 locus) were then transduced with each lentiviral vector and, 3 days later, selected with blasticidin (10 mg/mL) until > 80% of the cells were mCherry positive (6-9 days).

    Article Title: Large-scale mapping and mutagenesis of human transcriptional effector domains.
    Article Snippet: .. Individual recruitment assays and flow cytometry measurements Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker, using GoldenGate cloning in the backbone pJT126 (Addgene no. 161926). .. K562 citrine reporter cells were then transduced with each lentiviral vector and, 3 days later, selected with blasticidin (10 mg ml−1) until more than 80% of the cells were mCherry positive (6–9 days).

    Article Title: Large-scale mapping and systematic mutagenesis of human transcriptional effector domains
    Article Snippet: .. Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker, using GoldenGate cloning in the backbone pJT126 (Addgene #161926). ..

    Flow Cytometry:

    Article Title: Large-scale mapping and mutagenesis of human transcriptional effector domains.
    Article Snippet: .. Individual recruitment assays and flow cytometry measurements Protein fragments were cloned as a fusion with rTetR upstream of a T2A-mCherry-BSD marker, using GoldenGate cloning in the backbone pJT126 (Addgene no. 161926). .. K562 citrine reporter cells were then transduced with each lentiviral vector and, 3 days later, selected with blasticidin (10 mg ml−1) until more than 80% of the cells were mCherry positive (6–9 days).



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